東亞電子遊藝場-孫可
New Phytologist:生長素介導的信號級聯調控楊樹次生木質部發育Auxinmediated Aux/IAAARFHB signaling cascade regulates secondary xylem development in PopulusFirst author: Changzheng Xu; Affiliations: Southwest University (西南大學): Chongqing錛 ChinaCorresponding author: Keming LuoWood development is strictly regulated by various phytohormones and auxin plays a central regulatory role in this process. However錛 it remains obscure how the auxin signaling is transducted in developing secondary xylem during wood formation in tree species. Here錛 we identified an Aux/INDOLE3ACETIC ACID 9 (IAA9)AUXIN RESPONSE FACTOR 5 (ARF5) module in Populus tomentosa as a key mediator of auxin signaling to control early developing xylem development. PtoIAA9錛 a canonical Aux/IAA gene錛 is predominantly expressed in vascular cambium and developing secondary xylem and induced by exogenous auxin. Overexpression of PtoIAA9m encoding a stabilized IAA9 protein significantly represses secondary xylem development in transgenic poplar. We further showed that PtoIAA9 interacts with PtoARF5 homologs via the C erminal III/IV domains. The truncated PtoARF5.1 protein without the III/IV domains rescued the PtoIAA9m esulting defective phenotypes. Expression analysis showed that the PtoIAA9PtoARF5 module regulated the expression of genes associated with secondary vascular development in PtoIAA9m and PtoARF5.1overexpressing plants. Furthermore錛 PtoARF5.1 could bind to the promoters of two Class III homeodomainleucine zipper (HDIP III) genes PtoHB7 and PtoHB8 to modulate secondary xylem formation. Taken together錛 our results suggest that the Aux/IAA9ARF5 module is required for auxin signaling to regulate wood formation via orchestrating the expression of HDIP III transcription factors in poplar.木材的發育受到多個激素的調控,而生長素在其中扮演著一個非常重要的作用。然而,林木的木材形成過程中生長素在發育的次生木質部中的信號轉導還不是很清楚。本文中,作者在毛白楊中鑑定到了一個IAA9-ARF5模塊作為生長素信號轉導的核心調控子,控制木質部的早期發育。PtoIAA9屬于典型的Aux/IAA基因,主要在維管形成層和發育中的木質部中表達,並且受到外源生長素處理的誘導。過表達PtoIAA9m基因編碼一個穩定的IAA9蛋白顯著抑制轉基因楊樹中的次生木質部的發育。進一步的試驗顯示PtoIAA9與PtoARF5同源蛋白通過C末端III/IV結構域發生互作。將III/IV結構域截掉了的PtoARF5.1蛋白能夠拯救PtoIAA9m所導致的缺陷表型。表達分析顯示PtoIAA9PtoARF5模塊在PtoIAA9m和PtoARF5.1過表達植株中調控參與次生維管發育相關基因的表達。此外,PtoARF5.1能夠結合到兩個HDIP III基因PtoHB7和PtoHB8的啟動子區,進而調控次生木質部的形成。綜上,本文的研究揭示了楊樹中,Aux/IAA9ARF5模塊通過HDIP III轉錄因子作用于調控木材形成的生長素信號轉導。通訊:羅克明 (http://smkxxy.swu.edu.cn/viscms/smkxxyidex/jiaoshou3736/20151008/242191.html)個人簡介:1995C1999年,西南師範大學生命科學學院,學士;1999C2004年,西南農業大學與美國康涅狄格大學聯合培養,博士;2001C2003年,美國康涅狄格大學植物系,訪問學者;2007C2008年,美國密歇根理工大學生物技術中心,博士後。研究方向:以楊樹、擬南芥為模式植物,研究植物在幹旱、鹽堿、高光等逆境下適應性生長的分子機制和維管組織次生生長發育的遺傳調控機理,篩選和培育具有重要經濟價值和生態價值的林木新品種。doi: https://doi.org/10.1111/nph.15658Journal: New PhytologistFirst Published: 24 December錛 2018意大利火山引發地震:關注12月23-24日潮汐組合意大利4.8級地震造成多處房屋倒塌 至少28人受傷2018年12月27日13:47來源:中國新聞據歐聯網援引意大利歐聯通訊社報道,當地時間12月26日凌晨,位于埃特納火山附近的意大利西西裡大區卡塔尼亞省發生了多次地震,最大震級4.8級。地震造成多處房屋倒塌,至少有28名居民受傷。據報道,